Effect of elicitors on holm oak somatic embryo development and efficacy inducing tolerance to Phytophthora cinnamomi.
Identifieur interne : 000231 ( Main/Exploration ); précédent : 000230; suivant : 000232Effect of elicitors on holm oak somatic embryo development and efficacy inducing tolerance to Phytophthora cinnamomi.
Auteurs : M. Morcillo [Espagne] ; E. Sales [Espagne] ; L. Ponce [Espagne] ; A. Guillén [Espagne] ; J. Segura [Espagne] ; I. Arrillaga [Espagne]Source :
- Scientific reports [ 2045-2322 ] ; 2020.
Abstract
Holm oak trees (Quercus ilex L.) mortality is increasing worryingly in the Mediterranean area in the last years. To a large degree this mortality is caused by the oomycete Phytophthora spp., which is responsible for forest decline and dieback in evergreen oak forest areas of the southwestern Iberian Peninsula. This study is based on the possibility of applying chemical elicitors or filtered oomycete extracts to holm oak somatic embryos (SE) in order to induce epigenetic memory, priming, that may increase tolerance to the pathogen in future infections. To this end, we first examined the effect of priming treatments on SE development and its oxidative stress state, to avoid elicitors that may cause damage to embryogenic tissues. Both, the sterile oomycete extracts and the chemical elicitor methyl jasmonate (MeJA) did not produce any detrimental effect on SE growth and development, unlike the elicitors benzothiadiazole (BTH) and p-aminobenzoic acid (PABA) that reduced the relative weight gain and resulted in necrotic and deformed SE when were applied at high concentrations (25 µM BTH or 50 µM PABA) in accordance with their high malondialdehyde content. No significant differences among elicitation treatments were found in dual culture bioassays, although those SEs elicited with 50 µM MeJA increased H2O2 production after challenged against active oomycete indicating the activation of stress response. Since this elicitation treatment did not produce any adverse effect in the embryogenic process we suggest that could be used in further priming experiments to produce holm oak plants adapted to biotic stress.
DOI: 10.1038/s41598-020-71985-w
PubMed: 32938968
PubMed Central: PMC7495464
Affiliations:
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Arrillaga</name><affiliation wicri:level="1"><nlm:affiliation>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia, Spain. isabel.arrillaga@uv.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia</wicri:regionArea><wicri:noRegion>Burjassot Valencia</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Holm oak trees (Quercus ilex L.) mortality is increasing worryingly in the Mediterranean area in the last years. To a large degree this mortality is caused by the oomycete Phytophthora spp., which is responsible for forest decline and dieback in evergreen oak forest areas of the southwestern Iberian Peninsula. This study is based on the possibility of applying chemical elicitors or filtered oomycete extracts to holm oak somatic embryos (SE) in order to induce epigenetic memory, priming, that may increase tolerance to the pathogen in future infections. To this end, we first examined the effect of priming treatments on SE development and its oxidative stress state, to avoid elicitors that may cause damage to embryogenic tissues. Both, the sterile oomycete extracts and the chemical elicitor methyl jasmonate (MeJA) did not produce any detrimental effect on SE growth and development, unlike the elicitors benzothiadiazole (BTH) and p-aminobenzoic acid (PABA) that reduced the relative weight gain and resulted in necrotic and deformed SE when were applied at high concentrations (25 µM BTH or 50 µM PABA) in accordance with their high malondialdehyde content. No significant differences among elicitation treatments were found in dual culture bioassays, although those SEs elicited with 50 µM MeJA increased H<sub>2</sub>O<sub>2</sub> production after challenged against active oomycete indicating the activation of stress response. Since this elicitation treatment did not produce any adverse effect in the embryogenic process we suggest that could be used in further priming experiments to produce holm oak plants adapted to biotic stress.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32938968</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>09</Month><Day>16</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Effect of elicitors on holm oak somatic embryo development and efficacy inducing tolerance to Phytophthora cinnamomi.</ArticleTitle><Pagination><MedlinePgn>15166</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-020-71985-w</ELocationID><Abstract><AbstractText>Holm oak trees (Quercus ilex L.) mortality is increasing worryingly in the Mediterranean area in the last years. To a large degree this mortality is caused by the oomycete Phytophthora spp., which is responsible for forest decline and dieback in evergreen oak forest areas of the southwestern Iberian Peninsula. This study is based on the possibility of applying chemical elicitors or filtered oomycete extracts to holm oak somatic embryos (SE) in order to induce epigenetic memory, priming, that may increase tolerance to the pathogen in future infections. To this end, we first examined the effect of priming treatments on SE development and its oxidative stress state, to avoid elicitors that may cause damage to embryogenic tissues. Both, the sterile oomycete extracts and the chemical elicitor methyl jasmonate (MeJA) did not produce any detrimental effect on SE growth and development, unlike the elicitors benzothiadiazole (BTH) and p-aminobenzoic acid (PABA) that reduced the relative weight gain and resulted in necrotic and deformed SE when were applied at high concentrations (25 µM BTH or 50 µM PABA) in accordance with their high malondialdehyde content. No significant differences among elicitation treatments were found in dual culture bioassays, although those SEs elicited with 50 µM MeJA increased H<sub>2</sub>O<sub>2</sub> production after challenged against active oomycete indicating the activation of stress response. Since this elicitation treatment did not produce any adverse effect in the embryogenic process we suggest that could be used in further priming experiments to produce holm oak plants adapted to biotic stress.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Morcillo</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sales</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Departamento de Ciencias Agrarias y del Medio Natural, Escuela Politécnica Superior, Universidad de Zaragoza, Ctra Cuarte s/n, 22071, Huesca, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ponce</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guillén</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Segura</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arrillaga</LastName><ForeName>I</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>ISIC/ERI BIOTECMED, Departamento Biología Vegetal, Universidad de Valencia, Avda Vicent Andrés Estellés s/n, 46100, Burjassot Valencia, Spain. isabel.arrillaga@uv.es.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>06</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>17</Day><Hour>5</Hour><Minute>40</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32938968</ArticleId><ArticleId IdType="doi">10.1038/s41598-020-71985-w</ArticleId><ArticleId IdType="pii">10.1038/s41598-020-71985-w</ArticleId><ArticleId IdType="pmc">PMC7495464</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Methods Enzymol. 1990;186:421-31</Citation><ArticleIdList><ArticleId 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Morcillo</name></noRegion><name sortKey="Arrillaga, I" sort="Arrillaga, I" uniqKey="Arrillaga I" first="I" last="Arrillaga">I. Arrillaga</name><name sortKey="Guillen, A" sort="Guillen, A" uniqKey="Guillen A" first="A" last="Guillén">A. Guillén</name><name sortKey="Ponce, L" sort="Ponce, L" uniqKey="Ponce L" first="L" last="Ponce">L. Ponce</name><name sortKey="Sales, E" sort="Sales, E" uniqKey="Sales E" first="E" last="Sales">E. Sales</name><name sortKey="Segura, J" sort="Segura, J" uniqKey="Segura J" first="J" last="Segura">J. Segura</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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